Amylase Test

Reviewed

Also called: Amy test, serum amylase, urine amylase

Measures amylase enzyme levels to help diagnose or monitor pancreatic or salivary gland disorders.

Specimen: Blood, urine, or peritoneal fluid

Why this test may be ordered

  • To diagnose pancreatitis or other pancreatic disorders.
  • To monitor chronic pancreatitis, pancreatic cancer, cystic fibrosis, or other conditions affecting amylase levels.

Preparation and collection

Do not drink alcohol for 24 hours before the test. For a blood test, you may need to fast for 2 hours. Inform your provider about all medicines you take.

Blood sample from a vein, clean catch urine sample (or 24-hour urine collection), or peritoneal fluid sample via needle.

Blood test: slight pain or bruising at needle site. Urine test: no significant physical risks. Peritoneal fluid test: small risk of dizziness, bowel or bladder damage, bleeding, or infection.

Understanding results

Amylase levels are measured in blood, urine, or peritoneal fluid. Results are typically available within a few days. Your provider will interpret the results based on your overall health.

  • High amylase level: May indicate acute pancreatitis, a blocked pancreatic duct, or a pancreatic tumor. High levels in peritoneal fluid may indicate acute pancreatitis, blocked intestine, or reduced blood flow to the intestines. Limitation: High levels may also occur in other conditions; this result alone is not diagnostic. Next step: Your provider may order imaging or a lipase test to confirm the cause.
  • Low amylase level: May indicate chronic pancreatitis, kidney disease, cystic fibrosis, liver disease, or pre-eclampsia (in pregnancy). Limitation: Low levels are uncommon; this result alone is not diagnostic. Next step: Your provider may check kidney function, liver function, or test for cystic fibrosis.
  • Blood test: slight pain or bruising at needle site.
  • Urine test: no significant physical risks.
  • Peritoneal fluid test: small risk of bowel or bladder damage, bleeding, or infection.
  • False positives or negatives are possible; results should be interpreted in context with symptoms and other tests.

What to do next

Your healthcare provider will explain what your results mean and may recommend follow-up tests, such as a lipase test.

  • What do my amylase levels indicate about my pancreas or salivary glands?
  • Do I need additional tests, like a lipase test?
  • What follow-up care or monitoring is needed?

Evidence source

U.S. National Library of Medicine: Amylase Test

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